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leucovorin  (Merck KGaA)

 
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    Structured Review

    Merck KGaA leucovorin
    Leucovorin, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leucovorin/product/Merck KGaA
    Average 90 stars, based on 1 article reviews
    leucovorin - by Bioz Stars, 2026-02
    90/100 stars

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    a) Brightfield images of colon tumor organoids in hydrogel droplets on day 3, 6, 8 and 12 (scale bar 250 µm). b) Size development analysis of colon tumor organoids over several days, with average size highlighted above (n organoids < 110). c) Brightfield images (scale bar 250 µm) and H&E staining (scale bar 100 µm) of organoids grown in domes (1:6 dilution) or droplets. d) mIF staining of organoids in hydrogel droplets and domes (1:6 dilution) on day 12 (ECAD white, Ki67 red, SOX9 turquoise, CEACAM5 pink, scale bars 100 µm). e) Drug treatment of bioreactor grown tumor organoids. Dose-response curves to compounds FOLFOX (5-Fluorouracil,: <t>Leucovorin:Oxaliplatin</t> = 25:5:1, the concentration specified in the graph is based on 5-Fluorouracil), SN-38, Bortezomib, Capecitabine, quantified by fluorescence analysis of cell viability by live/dead staining (n samples = 3). Schematic was created with BioRender.com.
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    folinic acid leucovorin  (Millipore)
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    a) Brightfield images of colon tumor organoids in hydrogel droplets on day 3, 6, 8 and 12 (scale bar 250 µm). b) Size development analysis of colon tumor organoids over several days, with average size highlighted above (n organoids < 110). c) Brightfield images (scale bar 250 µm) and H&E staining (scale bar 100 µm) of organoids grown in domes (1:6 dilution) or droplets. d) mIF staining of organoids in hydrogel droplets and domes (1:6 dilution) on day 12 (ECAD white, Ki67 red, SOX9 turquoise, CEACAM5 pink, scale bars 100 µm). e) Drug treatment of bioreactor grown tumor organoids. Dose-response curves to compounds FOLFOX (5-Fluorouracil,: <t>Leucovorin:Oxaliplatin</t> = 25:5:1, the concentration specified in the graph is based on 5-Fluorouracil), SN-38, Bortezomib, Capecitabine, quantified by fluorescence analysis of cell viability by live/dead staining (n samples = 3). Schematic was created with BioRender.com.
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    Image Search Results


    a) Brightfield images of colon tumor organoids in hydrogel droplets on day 3, 6, 8 and 12 (scale bar 250 µm). b) Size development analysis of colon tumor organoids over several days, with average size highlighted above (n organoids < 110). c) Brightfield images (scale bar 250 µm) and H&E staining (scale bar 100 µm) of organoids grown in domes (1:6 dilution) or droplets. d) mIF staining of organoids in hydrogel droplets and domes (1:6 dilution) on day 12 (ECAD white, Ki67 red, SOX9 turquoise, CEACAM5 pink, scale bars 100 µm). e) Drug treatment of bioreactor grown tumor organoids. Dose-response curves to compounds FOLFOX (5-Fluorouracil,: Leucovorin:Oxaliplatin = 25:5:1, the concentration specified in the graph is based on 5-Fluorouracil), SN-38, Bortezomib, Capecitabine, quantified by fluorescence analysis of cell viability by live/dead staining (n samples = 3). Schematic was created with BioRender.com.

    Journal: bioRxiv

    Article Title: Integrated Microfluidic Platform for High-Throughput Generation of Intestinal Organoids in Hydrogel Droplets

    doi: 10.1101/2025.06.14.659486

    Figure Lengend Snippet: a) Brightfield images of colon tumor organoids in hydrogel droplets on day 3, 6, 8 and 12 (scale bar 250 µm). b) Size development analysis of colon tumor organoids over several days, with average size highlighted above (n organoids < 110). c) Brightfield images (scale bar 250 µm) and H&E staining (scale bar 100 µm) of organoids grown in domes (1:6 dilution) or droplets. d) mIF staining of organoids in hydrogel droplets and domes (1:6 dilution) on day 12 (ECAD white, Ki67 red, SOX9 turquoise, CEACAM5 pink, scale bars 100 µm). e) Drug treatment of bioreactor grown tumor organoids. Dose-response curves to compounds FOLFOX (5-Fluorouracil,: Leucovorin:Oxaliplatin = 25:5:1, the concentration specified in the graph is based on 5-Fluorouracil), SN-38, Bortezomib, Capecitabine, quantified by fluorescence analysis of cell viability by live/dead staining (n samples = 3). Schematic was created with BioRender.com.

    Article Snippet: The highest drug concentrations were chosen as follows: 1 μM Bortezomib (Tocris), 200 μM SN-38 (Tocris), 50 μM Capecitabine (Selleckchem) and the combinatorial FOLFOX with 50 μM 5-Fluorouracil (Tocris), 2 μM Oxaliplatin (Tocris), 10 μM Leucovorin (Selleckchem).

    Techniques: Staining, Concentration Assay, Fluorescence